Fig. 5. The mechanical regulation of MSC differentiation to adipocyte via cell spreading and YAP activation in single MSCs seeded onto micropatterned surfaces with controlled areas (300 to 10000 μm²). (A) Immunofluorescence imaging of YAP/TAZ (green) localization in MSC seeded onto micropatterned surfaces with controlled areas. (B) Immunofluorescence imaging of single MSC grown onto micropatterns with the increasing surface area for 3 days in adipogenic medium. Single-cell stained for lipids (green), F-actin (red), and nuclear (blue). (B) YAP/TAZ nuclear/cytoplasmic localization and (C) percentage of adipocytes for each area. Dashed lines indicate the adhesion area (Reprinted with permission, Elsevier, for citation see [57]).